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Human pre-mRNA 3'-end processing machinery database
CF IIm - mammalian cleavage factor II
It is build from two proteins which are conserved from yeast
to human. The
exact function is unknown. CFIIm in human is required for the cleavage
step, while in Drosophila and yeast it is required both for pre-mRNA 3'
processing and translational termination. Pcf11 binds to C terminal
part of RPB1 protein (this interacion depends on phosphorylation of
CTD). Although, Clp1 can acts as an RNA 5'-kinase it is not clear if
this activity is necessary for pre-mRNA 3' processing.
Literature:
- de Vries H, Ruegsegger U, Hubner W et al. Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors. EMBO J 2000 19(21):5895-904, doi: 10.1093/emboj/19.21.5895, PMID: 11060040.
- Noble CG, Beuth B, Taylor IA et al. Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor. Nucleic Acids Res 2007 35(1):87-99, doi: 10.1093/nar/gkl1010, PMID: 17151076.
- Ghazy MA, Gordon JM, Lee SD et al. The interaction of Pcf11 and Clp1 is needed for mRNA 3"-end formation and is modulated by amino acids in the ATP-binding site. Nucleic Acids Res 2012 40(3):1214-25, doi: 10.1093/nar/gkr801, PMID: 21993299.
- Mandel CR, Bai Y, Tong L et al. et al. Protein factors in pre-mRNA 3"-end processing. Cell Mol Life Sci 2008 65(7-8):1099-122, doi: 10.1007/s00018-007-7474-3, PMID: 18158581.
- Yang Q, Doublie S.. Structural biology of poly(A) site definition. Wiley Interdiscip Rev RNA 2011 2(5):732-47, doi: 10.1002/wrna.88, PMID: 21823232.
- Shi Y, Di Giammartino DC, Taylor D et al. et al. Molecular architecture of the human pre-mRNA 3" processing complex. Mol Cell 2009 33(3):365-76, doi: 10.1016/j.molcel.2008.12.028, PMID: 19217410.
Contact: Lukasz Kozlowski
This work was supported by Polish Ministry of Science and Higher Education (grant NN301 190139).
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