Home
Disorder
Domains
Protein interactions
Sub-complexes    CPSF
   CstF
   CFIm
   CFIIm
Other proteins    PABP-1
   PAPOLA
   RPB1
   PP-1A
   PP-1B
   Symplekin
   WDR82
   SSU72
   RBBP6
Download
FAQ

Symplekin


Known abbreviations: Symplekin, SPK, SYM, SYMPK

Yeast homolog: PTA1

Protein




Structures


Models
Domains

Secondary structure



NTD, CTD

Top 3 models:
Template: 3o2t, chain A
Method: hhsearch
Fragment: 3-395
Template: 3gs3, chain A
Method: hhsearch
Fragment: 14-288
Template: 2po4, chain A
Method: compass
Fragment: 92-817


This protein has alternative isoforms:
Isoform 1:
Sequence length:1274 aa
Molecular weight:141.148 kDa
Isoelectric point:5.88
Sequence:
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MASGSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAI
LTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSL
ANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQIS
GQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIKRRLSAQGQAISVVGSLSSMSPLEEEAPQAK
RRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFNSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGASGSLDKYEDCLIRLL
SGLQEKPDQKDGIFTKVVLEAPLITESALEVVRKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAA
PWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKL
IKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSVKCDMKSIIKATNLCFAERNVYTSEVLAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIK
CCQRTKPQSFQVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMTILEASGKQEPEAKEAPAGPLEEDDLEPLTLAPAPAPRPPQDLIGLRLAQEKALKRQLEEEQKLKPGGVGAPSSS
SPSPSPSARPGPPPSEEAMDFREEGPECETPGIFISMDDDSGLTEAALLDSSLEGPLPKETAAGGLTLKEERSPQTLAPVGEDAMKTPSPAAEDAREPEAKGNS



Isoform 2:
Sequence length:673 aa
Molecular weight:74.523 kDa
Isoelectric point:7.3
Sequence:
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MASGSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAI
LTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSL
ANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQIS
GQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIKRRLSAQGQAISVVGSLSSMSPLEEEAPQAK
RRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFNSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGASPRLCWRRHSSQRVP
WRWSASTARMRVAPIWACPHFET



Gene location: 19q13.3


Summary
Symplekin is a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Two separate isoforms are known. The first 964 residues of the two forms of symplekin are identical, suggesting that they are derived from alternative splicing. Symplekin and Pta1p share very weak sequence homology, but a putative conserved region between the two proteins have been identified by sequence analysis, with 17% identity and 31% similarity.

May have a house-keeping rule.

Found in a heat-sensitive complex at least composed of several cleavage and polyadenylation specific and cleavage stimulation factors.

Interacts with CPSF2, CPSF3 and CSTF2.
Interacts with HSF1 in heat-stressed cells.

It is detected in testis, expressed in polar epithelia and Sertoli cells but not in vascular endothelia. Additionally, the protein is detected in stomach, duodenum, pancreas, liver, fetal brain, carcinomas, lens-forming cells, fibroblasts, lymphocytes, lymphoma cells, erythroleukemia cells but not in endothelium of vessels, epidermis, intercalated disks, Purkinje fiber cells of the heart and lymph node.

Could be used as a differentiation marker in the differential diagnosis of tumors.

See this protein in other databases:
       

Literature:

Additional computationally mapped references ...


Previous
Polyribonucleotide 5'-hydroxyl...
Home Next
pre-mRNA 3' end processing pro...


Valid HTML 4.01!