 |
||||||||
|
||||||||
 |
||||||||
pre-mRNA 3' end processing protein WDR33
Known also as: WD repeat-containing protein 33, WD repeat-containing protein WDC146
Known abbreviations: WDR33, NET14, WDC146
Yeast homolog: PFS2
Protein
Models Domains Secondary structure NTD, CTD |
This protein is highly disordered (72 % of residues are predicted to be disordered)
Top 3 models:
 |  |  |
| Template: 1vyh, chain C Method: hhsearch Fragment: 1-405 | Template: 2po4, chain A Method: compass Fragment: 19-958,many gaps | Template: 4ery, chain A Method: blastp Fragment: 156-403 |
This protein has alternative isoforms:
Isoform 1:
| Sequence length: | 1336 aa |
| Molecular weight: | 145.891 kDa |
| Isoelectric point: | 9.49 |
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MATEIGSPPRFFHMPRFQHQAPRQLFYKRPDFAQQQAMQQLTFDGKRMRKAVNRKTIDYNPSVIKYLENRIWQRDQRDMRAIQPDAGYYNDLVPPIGMLNNPMNAVTTKFVRTSTNKVKCPVFVVRWTPE
GRRLVTGASSGEFTLWNGLTFNFETILQAHDSPVRAMTWSHNDMWMLTADHGGYVKYWQSNMNNVKMFQAHKEAIREASFSPTDNKFATCSDDGTVRIWDFLRCHEERILRGHGADVKCVDWHPTKGLVV
SGSKDSQQPIKFWDPKTGQSLATLHAHKNTVMEVKLNLNGNWLLTASRDHLCKLFDIRNLKEELQVFRGHKKEATAVAWHPVHEGLFASGGSDGSLLFWHVGVEKEVGGMEMAHEGMIWSLAWHPLGHIL
CSGSNDHTSKFWTRNRPGDKMRDRYNLNLLPGMSEDGVEYDDLEPNSLAVIPGMGIPEQLKLAMEQEQMGKDESNEIEMTIPGLDWGMEEVMQKDQKKVPQKKVPYAKPIPAQFQQAWMQNKVPIPAPNE
VLNDRKEDIKLEEKKKTQAEIEQEMATLQYTNPQLLEQLKIERLAQKQVEQIQPPPSSGTPLLGPQPFPGQGPMSQIPQGFQQPHPSQQMPMNMAQMGPPGPQGQFRPPGPQGQMGPQGPPLHQGGGGPQ
GFMGPQGPQGPPQGLPRPQDMHGPQGMQRHPGPHGPLGPQGPPGPQGSSGPQGHMGPQGPPGPQGHIGPQGPPGPQGHLGPQGPPGTQGMQGPPGPRGMQGPPHPHGIQGGPGSQGIQGPVSQGPLMGLN
PRGMQGPPGPRENQGPAPQGMIMGHPPQEMRGPHPPGGLLGHGPQEMRGPQEIRGMQGPPPQGSMLGPPQELRGPPGSQSQQGPPQGSLGPPPQGGMQGPPGPQGQQNPARGPHPSQGPIPFQQQKTPLL
GDGPRAPFNQEGQSTGPPPLIPGLGQQGAQGRIPPLNPGQGPGPNKGDSRGPPNHHMGPMSERRHEQSGGPEHGPERGPFRGGQDCRGPPDRRGPHPDFPDDFSRPDDFHPDKRFGHRLREFEGRGGPLP
QEEKWRRGGPGPPFPPDHREFSEGDGRGAARGPPGAWEGRRPGDERFPRDPEDPRFRGRREESFRRGAPPRHEGRAPPRGRDGFPGPEDFGPEENFDASEEAARGRDLRGRGRGTPRGGRKGLLPTPDEF
PRFEGGRKPDSWDGNREPGPGHEHFRDTPRPDHPPHDGHSPASRERSSSLQGMDMASLPPRKRPWHDGPGTSEHREMEAPGGPSEDRGGKGRGGPGPAQRVPKSGRSSSLDGEHHDGYHRDEPFGGPPGS
GTPSRGGRSGSNWGRGSNMNSGPPRRGASRGGGRGR
Isoform 2:
| Sequence length: | 326 aa |
| Molecular weight: | 38.294 kDa |
| Isoelectric point: | 9.77 |
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MATEIGSPPRFFHMPRFQHQAPRQLFYKRPDFAQQQAMQQLTFDGKRMRKAVNRKTIDYNPSVIKYLENRIWQRDQRDMRAIQPDAGYYNDLVPPIGMLNNPMNAVTTKFVRTSTNKVKCPVFVVRWTPE
GRRLVTGASSGEFTLWNGLTFNFETILQAHDSPVRAMTWSHNDMWMLTADHGGYVKYWQSNMNNVKMFQAHKEAIREARFIHNIPFSVVPIVMVKLFSKCILGAEMHGLCQFLGNFLHPINTIFFFVFTH
SPFCWHLSEVVLSRYQPLQYVRDVLSAAFCTGFLFSFMINNVYTLFLFIIYCVRQEYFIPNKEFSL
Isoform 3:
| Sequence length: | 257 aa |
| Molecular weight: | 30.288 kDa |
| Isoelectric point: | 9.83 |
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MATEIGSPPRFFHMPRFQHQAPRQLFYKRPDFAQQQAMQQLTFDGKRMRKAVNRKTIDYNPSVIKYLENRIWQRDQRDMRAIQPDAGYYNDLVPPIGMLNNPMNAVTTKFVRTSTNKVKCPVFVVRWTPE
GRRLVTGASSGEFTLWNGLTFNFETILQAHDSPVRAMTWSHNDMWMLTADHGGYVKYWQSNMNNVKMFQAHKEAIREASFSPTDNKFATCSDDGTVRIWDFLRCHEERILRDTCFHHCRCYFLSVKR
Gene location: 2q14.3
Summary
WDR33 is essential for both cleavage and polyadenylation of pre-mRNA 3' ends. Component of the cleavage and polyadenylation specificity factor (CPSF) module of the pre-mRNA 3' end processing complex.
Protein is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes.
Interacts with CPSF3/CPSF73.
Most highly expressed in testis and the protein is localized to the nucleus.
See this protein in other databases:
Literature:
- Ohnacker M, Barabino SM, Preker PJ et al. The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3"-end-processing complex. EMBO J 2000 19(1):37-47, doi: 10.1093/emboj/19.1.37, PMID: 10619842.
- Ito S, Sakai A, Nomura T et al. A novel WD40 repeat protein, WDC146, highly expressed during spermatogenesis in a stage-specific manner. Biochem Biophys Res Commun 2001 280(3):656-63, doi: 10.1006/bbrc.2000.4163, PMID: 11162572.
- Shi Y, Di Giammartino DC, Taylor D et al. Molecular architecture of the human pre-mRNA 3" processing complex. Mol Cell 2009 33(3):365-76, doi: 10.1016/j.molcel.2008.12.028, PMID: 19217410.
| Previous Symplekin |
Home | Next Serine/threonine-protein phosp... |
|
|
