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Human pre-mRNA 3'-end processing machinery databaseCPSF - cleavage and polyadenylation specificity factor CPSF is required for both cleavage and polyadenylation. It recognizes A(A/U)UAAA cassette and function
as scaffold for other components of complex. Although, the recognition is sequence specific, none of CPSF components posses standard RRM domain, even CPSF-160 which is responsible for this interaction.
At least two other components (Fip1 and CPSF30) also binds to RNA. Fip1 binds to U-rich cassete through C-terminal arginine-rich domain, while CPSF30 binding is mediated by zinc finger domain.
Recently it has been suggested that CPSF100, CPSF73 and additionally symplekin form the core of CPSF complex and other counterparts join the peripheria of the complex. CPSF73 is a endonuclease responsible for the cleavage step. It is zinc-dependent protein with weak activity stimulated by other factor of the complex. CPSF100 which is highly similar to CPSF73 lacks zinc-binding motif and therefore has no endonucleolytic activity. The function of CPSF100 is unknown. Literature:
Contact: Lukasz Kozlowski This work was supported by Polish Ministry of Science and Higher Education (grant NN301 190139). |
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