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Human pre-mRNA 3'-end processing machinery database
CPSF - cleavage and polyadenylation specificity factor |
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CPSF is required for both cleavage and polyadenylation. It recognizes A(A/U)UAAA cassette and function
as scaffold for other components of complex. Although, the recognition is sequence specific, none of CPSF components posses standard RRM domain, even CPSF-160 which is responsible for this interaction.
At least two other components (Fip1 and CPSF30) also binds to RNA. Fip1 binds to U-rich cassete through C-terminal arginine-rich domain, while CPSF30 binding is mediated by zinc finger domain.
Recently it has been suggested that CPSF100, CPSF73 and additionally symplekin form the core of CPSF complex and other counterparts join the peripheria of the complex.
CPSF73 is a endonuclease responsible for the cleavage step. It is zinc-dependent protein with weak activity stimulated by other factor of the complex. CPSF100 which is highly similar to CPSF73 lacks zinc-binding motif and therefore has no endonucleolytic activity. The function of CPSF100 is unknown.
Recently it has been suggested that CPSF100, CPSF73 and additionally symplekin form the core of CPSF complex and other counterparts join the peripheria of the complex.
CPSF73 is a endonuclease responsible for the cleavage step. It is zinc-dependent protein with weak activity stimulated by other factor of the complex. CPSF100 which is highly similar to CPSF73 lacks zinc-binding motif and therefore has no endonucleolytic activity. The function of CPSF100 is unknown.
Literature:
- Kaufmann I, Martin G, Friedlein A et al. et al. Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase. EMBO J 2004 23(3):616-26, doi: 10.1038/sj.emboj.7600070, PMID: 14749727.
- Sullivan KD, Steiniger M, Marzluff WF et al. et al. A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs. Mol Cell 2009 34(3):322-32, doi: 10.1016/j.molcel.2009.04.024, PMID: 19450530.
- Murthy KG, Manley JL.. The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3"-end formation. Genes Dev 1995 9(21):2672-83, doi: , PMID: 7590244.
- Mandel CR, Bai Y, Tong L et al. et al. Protein factors in pre-mRNA 3"-end processing. Cell Mol Life Sci 2008 65(7-8):1099-122, doi: 10.1007/s00018-007-7474-3, PMID: 18158581.
- Yang Q, Doublie S.. Structural biology of poly(A) site definition. Wiley Interdiscip Rev RNA 2011 2(5):732-47, doi: 10.1002/wrna.88, PMID: 21823232.
- Shi Y, Di Giammartino DC, Taylor D et al. et al. Molecular architecture of the human pre-mRNA 3" processing complex. Mol Cell 2009 33(3):365-76, doi: 10.1016/j.molcel.2008.12.028, PMID: 19217410.
Contact: Lukasz Kozlowski
This work was supported by Polish Ministry of Science and Higher Education (grant NN301 190139).
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