Home
Disorder
Domains
Protein interactions
Sub-complexes    CPSF
   CstF
   CFIm
   CFIIm
Other proteins    PABP-1
   PAPOLA
   RPB1
   PP-1A
   PP-1B
   Symplekin
   WDR82
   SSU72
   RBBP6
Download
FAQ

Cleavage and polyadenylation specificity factor subunit 4


Known also as: Cleavage and polyadenylation specificity factor 30 kDa subunit, CPSF 30 kDa subunit, NS1 effector domain-binding protein 1
Known abbreviations: CPSF4, CPSF30, NAR, NEB1

Yeast homolog: YTH1

Protein




Structures
Models
Domains

Secondary structure

Disorder

This protein is highly disordered (45 % of residues are predicted to be disordered)

Top 3 models:
Template: 2d9n, chain A
Method: hhsearch
Fragment: 52-130
Template: 2d9n, chain A
Method: phyre
Fragment: 30-95
Template: 2cqe, chain A
Method: hhsearch
Fragment: 52-86, 145-213


This protein has alternative isoforms:
Isoform 1:
Sequence length:269 aa
Molecular weight:30.255 kDa
Isoelectric point:8.12
Sequence:
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRG
FCKHGPLCRHRHTRRVICVNYLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKQSNNPPLQRSSSLIQLTSQNSSPNQQRTPQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTK
GHLAFLSGQ



Isoform 2:
Sequence length:244 aa
Molecular weight:27.547 kDa
Isoelectric point:8.03
Sequence:
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRG
FCKHGPLCRHRHTRRVICVNYLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKQRTPQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTKGHLAFLSGQ



Isoform 3:
Sequence length:243 aa
Molecular weight:27.419 kDa
Isoelectric point:8.03
Sequence:
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRG
FCKHGPLCRHRHTRRVICVNYLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKRTPQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTKGHLAFLSGQ



Gene location: 7q22.1


Summary
CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).

CPSF-30 is required for both cleavage and polyadenylation.

CPSF-30 has a second isoform in humans (locus XP_945726), sharing 54% sequence identity, but its function is not known.

CPSF-30 contains five CCCH zinc finger motifs (ZF1-ZF5) in all eukaryotes, and metazoan CPSF-30 has an additional CCHC zinc knuckle at the C-terminus. CCCH zinc fingers have the consensus sequence CX8CX5CX3H, while CCHC zinc knuckles have the consensus sequence CX2CX4HX4C. CCCH zinc finger motifs are involved in protein-protein interactions (e.g. ZF4 and ZF5 are required for interactions with Fip1p).

Association with influenza A virus NS1 blocks processing of pre-mRNAs, thereby preventing nuclear export of host cell mRNAs. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.

See this protein in other databases:
         

Literature:

Additional computationally mapped references ...


Previous
Cleavage and polyadenylation s...
Home Next
Pre-mRNA 3'-end-processing fac...


Valid HTML 4.01!