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Cleavage and polyadenylation specificity factor subunit 1


Known also as: Cleavage and polyadenylation specificity factor 160
Known abbreviations: CPSF1, CPSF160, HSU37012, P/cl.18

Yeast homolog: CFT1

Protein

1
1443




Domains

Secondary structure



NTD, CTD

Top 3 models:
Template: 3ei3, chain A
Method: hhsearch
Fragment: 1-990
Template: 3ei3, chain A
Method: pdbblast
Fragment: 2-629
Template: 3e0c, chain A
Method: compass
Fragment: 5-979


Sequence length:1443 aa
Molecular weight:160.884 kDa
Isoelectric point:6.14

Sequence:
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MYAVYKQAHPPTGLEFSMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDRSTEGKAHREKLELAASFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRD
GFVQNVHTPRVRVDPDGRCAAMLVYGTRLVVLPFRRESLAEEHEGLVGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLHGYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTS
LPFDCTQALAVPKPIGGVVVFAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQATFISYDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGN
SLLLKYTEKLQEPPASAVREAADKEEPPSKKKRVDATAGWSAAGKSVPQDEVDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCANAAVGEPAFLSEEFQNSPEPDLEIVVCSGHGKNGALSVLQKSIR
PQVVTTFELPGCYDMWTVIAPVRKEEEDNPKGEGTEQEPSTTPEADDDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPLGIRLLEGVNQLHFIPVDLGAPIVQCA
VADPYVVIMSAEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVITLCLYRDLSGMFTTESRLGGARDELGGRSGPEAEGLGSETSPTVDDEEEMLYGDSGSLFSPSKEEARRSSQPPADRDPAPFRAE
PTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEARREEATRQGELPLVKEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSK
KKAEGGGAEEGAGARGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMAIDGPVDSFAPFHNVNCPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATST
NTPCARIPRMTGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKG
PVTALCHCNGHLVSAIGQKIFLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRA
DFHVGAHVNTFWRTPCRGATEGLSKKSVVWENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLDGELLNRYLYLSTMERSELAKKIGTTPDIIL
DDLLETDRVTAHF


Gene location: 8q24.23


Summary
CPSF1 is the largest subunit of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal (PAS sequence) in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.

CPSF-160 is conserved throughout eukaryotes (24% identity and 51% similarity between CPSF-160 and Cft1p/Yhh1p)

Besides its role in 3?-end processing, it associates with factors involved in transcriptional initiation (TFIID) and elongation (PolII CTD), and plays a role in transcriptional termination.

Human CPSF-160 contains two RNP-type binding motifs near the N-terminus (RNP1, residues 379–386 and RNP2, residues 344–349), but it is not known whether these residues directly interact with RNA. A total of sixteen ?-propeller repeats were identified in human CPSF-160 based on sequence analysis.

Found in a complex with CPSF1, FIP1L1 and PAPOLA.
Interacts with FIP1L1, PAPD4/GLD2 and SRRM1.
Interacts with TUT1; the interaction is direct and mediates the recruitment of the CPSF complex on the 3'UTR of selected pre-mRNAs.

See this protein in other databases:
         

Literature:

Additional computationally mapped references ...


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