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FAQ

Cleavage stimulation factor subunit 2


Known also as: CF-1 64 kDa subunit, Cleavage stimulation factor 64 kDa subunit
Known abbreviations: CSTF2, CstF-64

Yeast homolog: RNA15

Protein




Structures
Models
Domains

Secondary structure

Disorder

This protein is highly disordered (79 % of residues are predicted to be disordered)

Top 3 models:
Template: 1cvj, chain A
Method: hhsearch
Fragment: 1-208
Template: 3dxj, chain N
Method: compass
Fragment: 1-476
Template: 2qfj, chain A
Method: phyre
Fragment: 2-176


This protein has alternative isoforms:
Isoform 1:
Sequence length:577 aa
Molecular weight:60.959 kDa
Isoelectric point:6.56
Sequence:
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISK
AVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQM
PAAVTGPGPGSLAPGGGMQAQVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGP
MLDQRGPPLDGRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTGMQGASIQGGSQP
GGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP



Isoform 2:
Sequence length:560 aa
Molecular weight:59.252 kDa
Isoelectric point:6.5
Sequence:
1........10........20........30........40........50........60........70........80........90........100.......110.......120.......130
MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISK
AVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGVPAPGQMPAAVTGPGPGSLAPGGG
MQAQVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDP
RGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEK
AALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP



Gene location: Xq22.1


Summary
CSTF2 is a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

CstF-64 contains a conserved RNA recognition motif (RRM) RNA binding domain (43% identity between CstF-64 and Rna15p) at its N-terminus. The RRM alone is sufficient for RNA binding and shows preference for the G/U- rich DSE. The structure of the RRM of CstF-64 has been determined by NMR, which showed the presence of a UU dinucleotide-specific binding site and that the protein:RNA interface is highly mobile. This flexibility may allow CstF-64 to form stable complexes with a wide range of GU-rich sequences.

Metazoan CstF-64 homologs contain additional sequences between the hinge region and the CTD, including a proline/glycine-rich segment followed by 12 repeats of the MEAR(A/G) pentapeptide motif. The length and composition of this region is variable among metazoans and its function is unknown. The MEAR(A/G) repeats may form a helical structure in solution.

CstF-64 has a second isoform in humans, known as tau CstF-64. It is expressed in male germ cells and may have important roles in germ-cell-specific polyadenylation and spermatogenesis.

CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1.

Interacts with HSF1 in heat-stressed cells.

Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1.

Up-regulated during the G0 to S phase transition. Phosphorylated upon DNA damage, probably by ATM or ATR.

See this protein in other databases:
         

Literature:

Additional computationally mapped references ...


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